COMPARISON OF GEN X-PERTS MTB/RIF ASSAY WITH THE CONVENTIONAL DIAGNOSIS (ZIEHL NIELSEN AND FLORESENCE) TO DETECT MTB POSITIVE AND UTTB AMONG PATIENTS VISITING UNIVERSITY OF GONDAR COMPREHENSIVE SPECIALIZEDHOSPITAL, NORTH WEST ETHIOPIA.

Abstract

Tuberculosis (TB) caused by Mycobacterium tuberculosis (MTB) remains a major global public health concern. Extra pulmonary TB accounts 15% of global TB burden and Ethiopia has reported a higher than average proportion of extra pulmonary tuberculosis (EPTB). Urinary tract TB (UTB) is one of the most common types of EPTB and is also considered as a severe form of EPTB in clinical practice. The diagnosis of UTTB is difficult the symptoms are similar to other bacterial infections, which serves as the major cause for delay diagnosis of UTTB patients. The data on diagnosis of urinary tracttuberculosis byGen X-pert MTB/RIF assayfrom developing countries including Ethiopia is yet limited due to constraints of resource and limitedaccesses ofGeneX-pert MTB/Rifampicin. The objective of this study was tocompare Gene X-pert MTB/RIF assay with convectional(Ziehl Nielsen and florescence microscopy) diagnosis for the detection of urinary tract tuberculosisfrom urine specimen among mycobacterium tuberculosis positive and suspected urinary tract tuberculosis casesat university of Gondar comprehensive specialized hospital, North West Ethiopia.A Hospital-based cross-sectional study was conducted among 64 sample with mycobacterium tuberculosis positive and suspected urinary tract tuberculosisat university of Gondar comprehensive specialized hospital. Non-randomized purposive sampling technique was used to select study participants. A urine sample was collected and smearmicroscopyZiehl Nielsen and florescence microscopy was done. A Gene XpertReal-time PCR was conducted to check the sample, hand free processing and DNA extraction platform. A total of 64 participants were enrolled in this study with response rate of 100%. Two third, 41 (64.1 %) were males and majority of the study participants were in the age group of 31-45 years, from majority of study participants 46 (71.9%) were from rural residence and 37(57.8%) were illiteratein education. Conclusion and recommendationthe prevalence of urinary tract mycobacterium tuberculosis by Gene X-pert and florescence microscopy 3(4.7%) and 1(1.6) which is slightly higher. The prevalence is higher in urban resident and female sex group. Gene X-pert more specific than convectional method. Develop guideline on how to use Expert in urine sample and diagnosis for UTTB