EVALUATION OF WOUND HEALING ACTIVITY OF 80% METHANOL
ROOT CRUDE EXTRACT AND SOLVENT FRACTIONS OF STEPHANIA
ABYSSINICA (Dill. & A. Rich.) Walp. (MENISPERMACEAE) IN MICE

dc.contributor.author	GOBEZIE, TESFAGEGN
dc.date.accessioned	2025-07-09T09:30:38Z
dc.date.available	2025-07-09T09:30:38Z
dc.date.issued	2025-07-09
dc.identifier.uri	http://hdl.handle.net/123456789/9640
dc.description.sponsorship	bstract Background: Medicinal plants have traditionally been used to heal a variety of human illnesses. The root of Stephania abyssinica (Dill. & A. Rich.) Walp. (Minispermaceae) is traditionally used to treat wounds. Despite the fact that there have been in vitro studies and claims supporting wound healing, there has been no scientific data on the in vivo wound healing activities of the root of Stephania abyssinica. Objective: The aim of the study was to evaluate the wound healing activity of 80% methanol root extract and solvent fractions of Stephania abyssinica in mice. Method: The roots of Stephania abyssinica were air dried, grounded and macerated by 80% methanol three times successively. The crude extract was fractionated by water, ethyl acetate and hexane. Both the crude extract and fractions were formulated as 5% w/w and 10% w/w ointment. The 2000 mg/kg of the 10% w/w crude extract was applied for acute dermal toxicity test. Wound healing activity of crude extract was evaluated on excision, incision and burn wound models, while the fractions were evaluated on excision wound model only. Wound healing was assessed by wound contraction, epithelialization period, tensile strength, and histological analysis. Results: In mice, an acute dermal toxicity test of 2000 mg/kg of the 10% w/w crude extract was found to be safe. Both 5% w/w and 10% w/w crude extract produced significant (p<0.001) wound contraction from 4th to 16th post wounding days, but the 5% w/w crude extract ointment were (p<0.01) wound contraction on 10th post wounding day as compared to simple ointment treated group on excision wound. On burn wound model the crude extract showed highly significant (p<0.001) from 6th post wounding days onwards and a significant (p<0.001) shortening period of epithelialization. The tensile strength was increased significantly (p<0.001) by the crude extract treated mice as compared to untreated group and negative control group. The aqueous and ethyl acetate fraction treated groups showed higher collagen deposition, fibroblast and blood cells as compared to the simple ointment treated mice in healed excision wound. Conclusion: The data obtained from this study showed 80% methanol crude extract, the aqueous and the 10% w/w EAF fractions possessed better wound healing activities, and decreased period of epithelialization	en_US
dc.language.iso	en	en_US
dc.subject	Wound healing, Stephania abyssinica, wound models, Ethiopia	en_US
dc.title	EVALUATION OF WOUND HEALING ACTIVITY OF 80% METHANOL ROOT CRUDE EXTRACT AND SOLVENT FRACTIONS OF STEPHANIA ABYSSINICA (Dill. & A. Rich.) Walp. (MENISPERMACEAE) IN MICE	en_US
dc.type	Thesis	en_US