Abstract:
ABSTRACT
Background: Traditionally, the root of Acacia seyal is used for the treatment of diarrhea.
However, its efficacy has not been scientifically evaluated. Thus, this study aimed to validate the
antidiarrheal activity using hydromethanolic crude extract and solvent fractions of Acacia seyal
roots in mice.
Methods: In all models, mice were randomly assigned into five groups of six mice each, and
group I (negative control) mice were received the vehicle, groups II, III, and IV mice were
received 100 mg/kg, 200 mg/kg, and 400 mg/kg doses of the extract, respectively. Group V
(positive control) mice were treated with loperamide 3 mg/kg for castor oil-induced diarrhea and
enteropooling test. For the gastrointestinal motility test, the mice were received atropine sulfate 5
mg/kg. Statistical significance of differences of both the number and weight of wet and total
feces, intestinal fluid accumulation, and distance traveled by a charcoal meal between groups
were analyzed by IBM SPSS version- 26.0 using one-way ANOVA followed by Tukey's HSD
post hoc multiple comparisons.
Result: In this study, the crude extract and n-hexane fraction significantly delayed the onset of
diarrhea, reduced both the weight and volume of intestinal contents as well as inhibited the
intestinal transit of charcoal meal at all tested doses as compared to the vehicle-treated group
(p<0.001). Similarly, the ethyl acetate fraction significantly (p<0.001) inhibited the weight of
intestinal content at all tested doses as compared to the negative control. While the aqueous
fraction statistically (p<0.01) prolonged the onset of diarrhea and inhibited gastrointestinal
motility only at the tested dose of 400 mg/kg. Amongst solvent fractions, the n-hexane fraction
achieved the highest anti- diarrheal index (106.51%).
Conclusion: According to this study, the hydromethanolic crude extract and solvent fractions of
Acacia seyal roots have promising antidiarrheal effects
